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. 2021 Oct 21;12:748497. doi: 10.3389/fimmu.2021.748497

Figure 2.

Figure 2

Rac1-siRNA, PLC-γ1-siRNA attenuated the effect of tryptophan (0.7mM) on transepithelial electrical resistance, permeability, and proinflammation cytokines (IL-8 and TNF-α) in ETEC K88-stimulated IPEC-J2 cells. About 70% confluent, IPEC-J2 cells were cultured in fetal bovine serum-free medium for 6 h and then transfected with 50 nM of NC-siRNA, Rac1-siRNA or PLCγ1-siRNA for 24 h, followed by treatment with tryptophan (0.7mM) for 48 h, and then treatment with ETEC K88 for 2h (2% FBS-medium without antibiotic, 1×108 CFU/mL). (A) Effect of ETEC K88 and Rac1-siRNA, PLC-γ1-siRNA on the transepithelial electrical resistance value. (B) Effect of ETEC K88 and Rac1-siRNA, PLC-γ1-siRNA on the permeability of FITC-dextran. (C) Effect of ETEC K88 and Rac1-siRNA, PLC-γ1-siRNA on the contents of IL-8. (D) Effect of ETEC K88 and Rac1-siRNA, PLC-γ1-siRNA on the contents of TNF-α. NC-siRNA was added to control, tryptophan and ETEC K88 groups. The TEER value and the permeability of all treatments were normalized to control. Data values are indicated as mean ± SEM (n = 3). Values with different letters indicate significant difference (P < 0.05).