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. 2021 Oct 21;12:748497. doi: 10.3389/fimmu.2021.748497

Figure 7.

Figure 7

Effect of pcDNA3.1-p(CaSR), Rac1-siRNA, PLC-γ1-siRNA and ETEC-K88 on transepithelial electrical resistance and permeability in IPEC-J2 cells. The IPEC-J2 cells were transfected with pcDNA3.1-p(CaSR), NC-siRNA, Rac1siRNA or PLC-γ1 siRNA for 48 h, followed by ETEC K88 (2% fetal bovine serum DMEM/F12 medium without antibiotic, 1×108 CFU/mL) for 2h. In control and ETEC K88 groups, cells were transfected with NC-siRNA and pcDNA3.1(+). In pcDNA3.1-CaSR-WT+ ETEC K88 groups, cells were transfected with NC-siRNA. (A) Effect of ETEC K88, pcDNA3.1-p(CaSR), Rac1siRNA and PLC-γ1 siRNA on the transepithelial electrical resistance value after stimulation with ETEC K88. (B) Effect of ETEC K88, pcDNA3.1-p(CaSR), Rac1-siRNA and PLC-γ1-siRNA on the permeability of FITC-dextran after stimulation with ETEC K88. The TEER value and the permeability of FITC-dextran of all treatments were normalized to control. Data values are expressed as mean ± SEM (n = 3). Values with different letters indicate significant difference (P < 0.05).