TABLE 1.

DOPE is preferentially situated on the inner leaflet of vesicles. $f_o$ corresponds to the fraction of the outer leaflet that is occupied by PE lipids whereas $f_i$ corresponds to the fraction of the inner leaflet that is occupied by PE lipids. The theoretical values are obtained thanks to curvature energy calculations. The experimental values are obtained thanks to the comparison of the fluorescence intensities of a sample after external PE labeling followed by total PE labeling. The errors correspond to the standard error on the mean of the different experiments. The ratio between the PE occupation of the outer leaflet compared to that of the inner leaflet confirms that there is an enrichment of PE on the inner leaflet.

DOPE input fraction ( $f$ )	DOPE fraction in the inner leaflet ( $f_i$ )		DOPE fraction in the outer leaflet ( $f_o$ )		Ratio between the outer and inner leaflets ( $r$ )
	Model	Experiment	Model	Experiment	Model	Experiment
0.25	0.37	0.42 ± 0.01	0.13	0.10 ± 0.01	0.35	0.24 ± 0.04
0.5	0.74	0.84 ± 0.02	0.26	0.27 ± 0.02	0.35	0.33 ± 0.03