Table 1.

Primers used in the study.

Primer name (purpose)	Sequence
hCaspase-1 D103N (mutate D103 to N)	For: 5’-GAAGAAAGTACTCCTTGAGAGTTTTGCATATTAAGGTAATTTCCA-3’ Rev: 5’-TGGAAATTACCTTAATATGCAAAACTCTCAAGGAGTACTTTCTTC-3’
hCaspase-1 D119N (mutate D119 to N)	For: 5’-GCATAGCTGGGTTGTTCTGCACTGCCTGAGG-3’ Rev: 5’-CCTCAGGCAGTGCAGAACAACCCAGCTATGC-3’
hCaspase-1 D297N (mutate D297to N)	For: 5’-CCAGAAACTCCTACTGAATTTTTAAACCACACCACACC-3’ Rev: 5’-GGTGTGGTGTGGTTTAAAAATTCAGTAGGAGTTTCTGG-3’
hCaspase-1 D314/315N (mutate D314/315 to N)	For: 5’-GTGGGCTTTCTTAATAGCATTATTCTCAAACTCTTCTGTAGTTG-3’ Rev: 5’-CAACTACAGAAGAGTTTGAGAATAATGCTATTAAGAAAGCCCAC-3’
hCaspase-1 DEAD (mutate C285 to A)	For: 5’-GCTGTCACCACGGGCGGCCTGGATGATG-3’ Rev: 5’-CATCATCCAGGCCGCCCGTGGTGACAGC-3’
GFP-EcoRI/BamHI (clone GFP in LAMP1-RFP)	For: 5’-CCCAAGCCCAGGAAGGGGCAGGTTCAGATAATGCACGTGTTT-3’ Rev: 5’-CCTATGGATCCATCAGCTACATACTTGTACAGCTCGTCCATGCCGAG-3’
GFP-YVAD-RFP (introduction of YVAD to GFP-RFP construct)	For: 5’-CTGATGGATCCACCGGCGCCACCATGGCCTCC-3’ Rev: 5’-GGAGGCCATGGTGGCGCCGGTGGATCCATCAG-3’