FIGURE 3.

N-terminal residues 1–20 of PCV4 Cap are essential for binding to NPM1. (A,B) HEK293T cells were co-transfected with plasmids containing full-length PCV4 Cap or truncated mutants, together with the Flag-NPM1 plasmid for 48 h; the cell lysate extracts were immunoprecipitated with Flag beads (A) or anti-GFP purified IgG (B) and then detected by western blotting using the indicated antibodies.