Figure 3.

Effect of HS on jejunal expression of GLUT1, GLUT2, and GLUT3 in JF, ACRB, 95RAN, and MRB birds. Protein and RNA were extracted from tissue and analyzed by western blot (A–D) and qPCR (E–G), respectively. Gene expression data are mean ± SEM (n = 6/group). Protein expression was normalized to loading via Ponceau stain (PS) (A). Protein was analyzed via AlphaView software and is expressed as mean ± SEM (n = 4/group) with one representative blot shown (A). Different letters indicate the significant difference at P < 0.05. GLUT, glucose transporter; HS, heat stress; TN, thermoneutral; JF, jungle fowl; ACRB, Athens Canadian Random Bred; 95RAN, 1995 random bred; MRB, modern random bred.