Figure 6.

Amino acid transporter protein expression in the jejunum of JF, ACRB, 95RAN, and MRB birds under TN and HS and in IPEC-J2 cell line. Protein was extracted from tissue and cells exposed to HS (45°C) or to 37°C (control) and analyzed by western blot (A–D). Protein expression was normalized to loading via Ponceau stain (PS) (A,C). Protein was analyzed via AlphaView software and is expressed as mean ± SEM (n = 4/group) for IPEC-J2 cells and (n = 4/group) for tissue. *indicate a significant difference compared to TN conditions for bird populations or 37°C for IPEC-J2 cell line at P < 0.05. HS, heat stress; TN, thermoneutral.