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. 2021 Oct 21;12:733134. doi: 10.3389/fphys.2021.733134

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Copyright © 2021 Abdelli, Ramser, Greene, Beer, Tabler, Orlowski, Pérez, Solà-Oriol, Anthony and Dridi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HS on the expression of fatty acid transporters in the jejunum of JF, ACRB, 95RAN, and MRB birds and in IPEC-J2 cell line. Protein and RNA were extracted from jejunal tissue and analyzed by western blot (A,B) and qPCR (C,D), respectively. Protein from cells was also analyzed by immunoblot (E,F). Gene and protein expression data are mean ± SEM (n = 4–6/group). Protein expression was normalized to loading via Ponceau stain (PS). Protein was analyzed via AlphaView software and one representative blot is shown. Different letters indicate a significant difference at P < 0.05 when the interaction effect is significant. CD36, the cluster of differentiation 36; FABP, fatty acid-binding protein; HS, heat stress; TN, thermoneutral; JF, jungle fowl; ACRB, Athens Canadian Random Bred; 95RAN, 1995 random bred; MRB, modern random bred.