Table 1.
Oligonucleotide real-time qPCR primers.
| Gene | Accession numbera | Primer sequence (5′ → 3′) | Orientation | Product size (bp) |
|---|---|---|---|---|
| GLUT1 | NM_205209.1 | TCCTGATCAACCGCAATGAG | Forward | 60 |
| TGCCCCGGAGCTTCTTG | Reverse | |||
| GLUT2 | NM_207178.1 | GAAGGTGGAGGAGGCCAAA | Forward | 61 |
| TTTCATCGGGTCACAGTTTCC | Reverse | |||
| GLUT3 | NM_205511.1 | TTGGGCGCTTCATTATTGG | Forward | 68 |
| CTCACTGATGTACATGGGAACAAAG | Reverse | |||
| GLUT5 | XM_040689119.1 | CCTCAGCATAGTGTGTGTCATCATT | Forward | 62 |
| GGATCGGACTGGCTCCAA | Reverse | |||
| GLUT8 | XM_040648927.1 | GCTGCCTCAGCGTGACTTTT | Forward | 58 |
| AGGGTCCGCCCTTTTGTT | Reverse | |||
| GLUT9 | XM_040670183.1 | CAGTGGATGAAAGCACCTTGAC | Forward | 63 |
| CACCGATGGCAAAAATGGA | Reverse | |||
| GLUT10 | XM_040688610.1 | AACGCAGAACAAAGATTCCTGAA | Forward | 65 |
| GTCATTCCACGTGCCAGCTT | Reverse | |||
| GLUT11 | NM_001347709.1 | CCCTCATCCAGCTCATGATTCT | Forward | 67 |
| CCACGGTCAATCAAGAGGTATCT | Reverse | |||
| GLUT12 | XM_040667840.1 | TTTGTGGACCTGTTTCGTTCAA | Forward | 61 |
| GCGTGAGCCCTACCAGCAT | Reverse | |||
| GLUT14 | XM_040672375.1 | TTGGGCGCTTCATTATTGG | Forward | 68 |
| CTCACTGATGTACATGGGAACAAAG | Reverse | |||
| SGLT1 | NM_001293240.1 | AGCATTTCAGCATGGTGTGTCT | Forward | 64 |
| TGCTCCTATCTCAGGGCAGTTC | Reverse | |||
| SLC1A4 | XM_040666934.1 | CGACTGATGAACAACGCAGAA | Forward | 119 |
| (ASCT1) | TCGCCAACCTCCGCATT | Reverse | ||
| SLC3A1 | XM_040667709.1 | CCTGGGCTGTGAGAAACCAA | Forward | 63 |
| (NBAT) | GGCACAAATTGAGTAGGAAGAAGAG | Reverse | ||
| SLC6A14 | XM_040670974.1 | GCTTCCGTGGTCAGATTGCT | Forward | 66 |
| TCATTTACGAGGCGTGTTTTACTG | Reverse | |||
| SLC6A19 | XM_040663289.1 | CGCTGGTGTGCCTAGTTTGA | Forward | 64 |
| ACAGCAATTTCTGATGGCTTTG | Reverse | |||
| SLC6A20 | XM_418798.6 | GCTGTCAAACCCCAAAACGT | Forward | 63 |
| (SIT1) | CCCAGACCCAGTGAGAAGAAGA | Reverse | ||
| SLC7A1 | NM_001145490.1 | AAAACTCCAGTAATTGCAACAGTGA | Forward | 68 |
| (CAT1) | AAGTCGAAGAGGAAGGCCATAA | Reverse | ||
| SLC7A2 | NM_001199102.1 | AGCTCTCCATCCACCATGTTG | Forward | 58 |
| (CAT2) | CCAGGCACCGAACAAAGGT | Reverse | ||
| SLC7A6 | XM_040681081.1 | CACGTGGGTGGCTTTGC | Forward | 61 |
| (Y + LAT2) | GAATTCTCCACGGCTCTGAACT | Reverse | ||
| SLC7A9 | NM_001199133.1 | GCTGTGGGTCCTTGTTTAACCA | Forward | 60 |
| (BAT1) | TGCACCTAGTGTTGCCAGAACT | Reverse | ||
| SLC7AL | XM_040665181.1 | GCTGAGTTGGGAGCATCCA | Forward | 66 |
| ACCAAACGCTTCCAGGATGT | Reverse | |||
| SLC38A2 | NM_001305439.1 | TGGCATCCTGGGACTTTCC | Forward | 66 |
| (SNAT2) | AGCAGGAGTATCACAAAAAGAGCAA | Reverse | ||
| LAT1 | NM_001030579.2 | CTGCTGCCGCCTGAGAA | Forward | 62 |
| CGCCGGCAGGAATTCC | Reverse | |||
| EAAT3 | XM_424930.7 | GGTGAAGGCGGACAGGAA | Forward | 68 |
| TGCTGAGCAGGAGCCAGTT | Reverse | |||
| PepT1 | NM_204365.1 | GACAACTTTTCTACAGCCATCTACCA | Forward | 65 |
| CCCAGGATGGGCGTCAA | Reverse | |||
| PepT2 | NM_001319028.1 | TGAAAAACCGCTCCCATCA | Forward | 61 |
| TGTTCCGATGCCCAGTCAA | Reverse | |||
| iFABP | NM_001007923.1 | CGTACCATCGACATCGAATTCA | Forward | 61 |
| TCCCGTCAGCCAGACTGTATT | Reverse | |||
| CD36 | XM_040686380.1 | ACTGCGCTTCTTCTCCTCTGA | Forward | 68 |
| TCACGGTCTTACTGGTCTGGTAAA | Reverse | |||
| 18s | AF173612 | TCCCCTCCCGTTACTTGGAT | Forward | 60 |
| GCGCTCGTCGGCATGTA | Reverse |
GLUT, glucose transporter; qPCR, quantitative PCR.