Table 4.
Primer design for genes analyzed by real-time PCR.1
| Target gene | Nucleotide sequence of primers (5'-3') | Product length, bp | Accession number | |
|---|---|---|---|---|
| Beta-actin | F: | GAGAAATTGTGCGTGACATCA | 152 | L08165 |
| R: | CCTGAACCTCTCATTGCCA | |||
| GAPDH | F: | ATGGCATCCAAGGAGTGA | 141 | NM 204,305.1 |
| R: | GGGAGACAGAAGGGAACAG | |||
| CYP7A1 | F: | GAT CTT CCC AGC CCT TGT GG | 82 | AY700578 |
| R: | AGC CTC TCC CAG CTT CTC AC | |||
| LXR-α | F: | GAC CTG AGC TAT AAT CGG GAT G | 255 | AF492498 |
| R: | TCA GGT GAT CAT TTG GTC TGT TG | |||
| FXR | F: | AGT AGA AGC CAT GTT CCT CCG TT | 182 | AF492497 |
| R: | GCA GTG CAT ATT CCT CCT GTG TC | |||
| SREBP-1 | F: | CAT TGG GTC ACC GCT TCT TCG TG | 236 | AY029224 |
| R: | CGT TGA GCA GCT GAA GGT ACT CC | |||
| SREBP-2 | F: | ACA GAC GCC AAG ATG CAC AAG TC | 339 | AJ414379 |
| R: | CCA CAG GAG GAG AGT CAG GTT CA |
GAPDH = glyceraldehyde-3-phosphate dehydrogenase; CYP7A1 = cholesterol 7-alpha hydroxylase; LXR-α = liver X receptor α; FXR = farnesoid X receptor; SREBP = sterol regulatory element binding protein.
1
Accession number refer to NCBI.