Table 1.
Solvent |
Crude Extracts |
Washing Fractions |
Elution Fractions |
|||||
---|---|---|---|---|---|---|---|---|
Yield (%) |
Water |
42.24 |
33.16 |
5.68 |
||||
Ethanol |
31.88 |
24.36 |
4.72 |
|||||
Recovery (%)b | Recovery (%) | Recovery (%) | ||||||
Phenolic Content | Content in 1 g Peel (µg GAEa) | Water | 6242.80 ± 198.56 | 100 | 2822.00 ± 103.52 | 45.20 | 3253.87 ± 153.60 | 52.12 |
Content in 1 g Peel (µg GAE) | Ethanol | 4244.80 ± 222.33** | 100 | 1752.53 ± 24.49 | 41.29 | 2457.33 ± 127.27 | 57.89 | |
Flavonoid Content | Content in 1 g Peel (µg REa) | Water | 3149.78 ± 1.54 | 100 | 243.17 ± 39.98 | 7.72 | 2470.22 ± 36.59 | 78.43 |
Content in 1 g Peel (µg RE) | Ethanol | 2080.72 ± 35.18*** | 100 | 183.04 ± 30.16 | 8.80 | 1749.42 ± 21.34 | 84.08 |
Data are presented as means ± SD of triplicate samples. * represents significant differences compare ethanol crude extract to water crude extract. ***, p ≤ 0.0001; **, p ≤ 0.001; *, p ≤ 0.05; ns, p > 0.05.
a) GAE, gallic acid equivalent; RE, rutin equivalent.
b) Recovery (%), the percentage of each fraction recovered from crude extracts.