Figure 3.

The functional conversion of TH17 cells into T cells with the immune-suppressive phenotype. (A) In the TH17-skewing condition, the change of IL-17A or Foxp3 expression in TH17 cells treated with ntHIF-1α-TMD were analyzed by flow cytometry. (B) The amount of IL-10 in the supernatant from T cells in (A) was measured by ELISA. (C, D) The immune-suppressive function of TH17 cells treated with ntHIF-1α-TMD was evaluated by suppression assay. (E) Expression heatmap of helper T cell-related genes. Z-score was calculated from log2(TPM+1). (F) GSEA-based enrichment plots of the Foxp3 targets cluster or TGF-β signaling pathway. Experiments in (A–D) were performed three times. Data are represented as mean ± SEM (n ≥ 3), and Student’s t-test was performed for statistical analysis. ns, not significant, *P < 0.05, **P < 0.01.