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Effect of ToAP2D treatment on apoptosis of S. globosa. (A) Laser scanning confocal microscope was used to observe the apoptosis and necrosis effect of ToAP2D treatment. The scale bar = 20 μm. (B) Flow cytometry was used to detect the ROS accumulation. (C) Flow cytometry was used to detect the mitochondrial membrane potential. (D) Flow cytometry was used to detect the metacaspase activation. *p < 0.05, **p < 0.01, and ***p < 0.001 indicate statistical significance.
