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. 2000 May;20(10):3705–3714. doi: 10.1128/mcb.20.10.3705-3714.2000

FIG. 2.

FIG. 2

FIG. 2

Deficient UV photoproduct repair in genomic DNA isolated from p53- or gadd45-null MEF lines. (A) Kinetics of 6-4 pp removal in MEFs determined by immunoassays following 10 J of UV irradiation m−2. Data were from two independent experiments conducted in triplicate. The values shown are means ± standard deviations. Note that 100% repair was defined relative to unirradiated cells. The bulk of the damage is repaired in wild-type cells within the first 3 to 4 h (compare the 4-h time point with the UDS data in Fig. 1). (B) Strand-specific repair of CPDs within the dhfr gene measured by TEV assays following 10 J of UV irradiation m−2. Repair of the NTS (red) was markedly reduced in gadd45−/− or gadd45/p21-null MEFs. Each of the MEF lines exhibited near normal TCR repair of the TS (blue).