FIGURE 3.

Hsa_circ_0001666 suppresses the proliferation and metastasis of CRC cells in vivo. (A–C) 1 × 10⁶ treated LoVo and DLD‐1 cells were injected subcutaneously into nude mice. After a month, the tumours were dissected and photographed. Tumour volume was measured as (length × width²)/2. (D) IHC revealed relative Ki‐67 expression levels in subcutaneous tumour tissues. (E) 1 × 10⁶ treated LoVo cells and DLD‐1 cells were, respectively, injected into the spleen and ileocolic vein of nude mice. Mice were then sacrificed and the lungs, and livers were stained by H&E. The number of metastases was calculated. Data were represented as mean ± SD (n = 3). **p < .01. ***p < .001