FIGURE 5.

Hsa_circ_0001666 suppresses CRC progression partially depending on miR‐576‐5p. LoVo cells were transfected with NC, si‐circ, si‐circ+miR‐inhibitor, and DLD‐1 cells were transfected with Vector, oe‐circ, oe‐circ+miR‐mimics. (A–C) To test the proliferative ability, CCK‐8 assays, colony formation assays, and EdU assays were used. (magnification, 200×; scale bar, 100 μm). (D) The Annexin‐V FITC/PI staining was used to assess the apoptotic rates. (E, F) Wound healing assays and transwell assays were used to determine cell migratory and invasive capabilities. The scale bar in wound healing assays indicated 20 μm; the scale bar in transwell assays indicated 200 μm. Data were all represented as mean ± SD (n = 3). n.s indicated no significance, *p < .05, **p < .01