FIGURE 6.

PCDH10 is a direct target of miR‐576‐5p in CRC. (A) Overlapped target genes of miR‐576‐5p predicted by TargetScan, StarBase, miRWalk, miRDB, TCGA. (B) Pearson correlation analysis of miR‐576‐5p with PCDH10/EPHA7/FOXP2 in StarBase. (C) Relative expression of three candidate mRNAs in treated LoVo and DLD‐1 cells by qPCR. (D) Relative expression of PCDH10 in treated LoVo and DLD‐1 cells by Western blot. (E) Relative expression of PCDH10 in CRC tissues and matched adjacent normal tissues (n = 70). (F, G) Pearson correlation analysis of miR‐576‐5p with PCDH10, and hsa_circ_0001666 with PCDH10 in CRC tissues. (H) After co‐transfection with PCDH10 3′UTR‐WT or PCDH10 3′UTR‐MUT and miR‐576‐5p mimics or mimics NC, relative luciferase activities were observed in HEK‐293T cells. (I) Several markers of the Wnt/β‐catenin signalling pathway were tested by Western blot in LoVo cells transfected with NC, si‐circ, miR‐inhibitor and si‐circ+miR‐inhibitor and DLD‐1 cells transfected with Vector, oe‐circ, miR‐mimics and oe‐circ+miR‐mimics. Data were all represented as mean ± SD (n = 3); n.s indicated no significance, *p < .05, **p < .01