FIGURE 2.

SMYD2 positively regulates the NF‐κB signaling cascade. Fibroblast‐like synoviocytes (FLS) were transfected with SMYD2 siRNA oligos or pCDH‐SMYD2, then treated with TNF‐α for 1 h and 2 h, cell lysates were prepared and blotted with phosphospecific antibodies to p65 and MAPK (A); Nuclear and cytoplasm distribution of p65 were examined by western blot (B); The cells were immunostained with anti‐p‐p65 and analyzed by fluorescence microscopy; scale bars: 100 μm (C). (D‐E) 293T cells were transfected with an appropriate amount of luciferase plasmids and treated with TNF‐α for 6 h, luciferase assay was performed to analyze NF‐κB transcriptional activity. Data are presented as mean ± S.D, n = 3, *p < 0.05, ***p < 0.001 vs TNF‐α group. (F) SMYD2 contributed to the expressions of COX2 and iNOS