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. 2021 Oct 21;12:734614. doi: 10.3389/fpls.2021.734614

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Copyright © 2021 Li, Zhang, Li, Chen, Yang, Zhang, Wang, Li, Reynolds, Jing, Mao and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TaWRKY51 specifically locates in the nucleus of the wheat protoplast. The constructs 35S::TaWRKY51-green fluorescent protein (GFP) and 35S::GFP were transformed into wheat protoplast by polyethylene glycol (PEG)-mediated method respectively. 1–3, wheat protoplast expressing GFP as a control. 4–6, wheat protoplast expressing TaWRKY51-GFP fusion protein. The pictures were taken in bright and black fields 36 h after transformation by a laser scanning confocal microscope.