Table 1.
The primers used in the current study for various purposes.
| Primer | Primer sequence (5′-3′) | Purpose |
|---|---|---|
| GAsF | GAGCACCTATCTTATCTTGGGAG | Isolating the genomic fragment of TaWRKY51-2A |
| GAsR | GTCACGAAGGGAACATGGTC | |
| GBsF | CGGTAAGCCCGTGTCTCTG | Isolating the genomic fragment of TaWRKY51-2B |
| GBsR | TAAACTGATGCGGCCAAAAG | |
| GDsF | AGCACCTATCTTATCTTGGGAG | Isolating the genomic fragment of TaWRKY51-2D |
| GDsR | CTAAGTTGAATTAACATGGCGA | |
| PAsF | TCTGTGTTAGAGTCTCCGAGCGT | Amplifying the promoter region of TaWRKY51-2A |
| PBsF | GCAACAGGCAATTAACGTAGTGAC | Amplifying the promoter region of TaWRKY51-2B |
| PDsF | CTGTGTTAGTGTCTTCGGGCG | Amplifying the promoter region of TaWRKY51-2D |
| PsR1 | TCCTCCAATCAGATCCATGGTC | Amplifying the promoter region of TaWRKY51-2B |
| PsR2 | CCCTCCCGTATCCTCCAATC | Amplifying the promoter regions of TaWRKY51-2A and -2D |
| Sub-F | CTggtaccATGGAGCAGAAACTCATCTCTGAAGAGGATATGATGACCATGGATCT (Kpn I) | Subcloning TaWRKY51 into binary vector pCAMBIA1391 |
| Sub-R | CTactagt TCAGAGCTCCGGGAGCGGGGCGAG (Spe I) | |
| J163-F | AGgagctcATGATGACCATGGATCT (Sac I) | Constructing subcellular localization vector |
| J163-R | AGagatctGAGCTCCGGGAGCGGGGCGAG (BglII) | |
| M13F | TGTAAAACGACGGCCAGT | Sequencing primers for pEASY-Blunt vectr |
| M13R | CAGGAAACAGCTATGACC | |
| APseqR1 | GTGACCCACCTGTCAGAGACT | Sequencing the promoter of TaWRKY51-2A |
| BSeq F1 | AAGGTGTACCAATTCCATCAT | Sequencing the promoter of TaWRKY51-2B |
| BSeq F2 | TAGTTAGGAGTAGAGTTTGTCAT | |
| DSeq F1 | CTTTGCTTTGTTGTTTCTTCATTC | Sequencing the promoter of TaWRKY51-2D |
| DSeq F2 | CGGCAGAACTGGAGGAGGTGTATC | |
| DSeq R1 | GGTGCTGTGCTGGTCTCG | |
| qRT-AF | TTAATTCAACTTAGCTTATG | Determining the expression of TaWRKY51-2A |
| qRT-AR | TCAAAGCTTCCAACTACACA | |
| qRT-BF | TGTTAATTCAACTTAGCTTG | Determining the expression of TaWRKY51-2B |
| qRT-BR | CATATTTTTCTTTCTCCTTC | |
| qRT-DF | GCCATTACCGTCGTCCTCGC | Determining the expression of TaWRKY51-2D |
| qRT-DR | AAGCTTCCAACTACACTTGC | |
| TaActin-qF | CTCCCTCACAACAACAACCGC | Quantifying gene expression in wheat |
| TaActin-qR | TACCAGGAACTTCCATACCAAC | |
| OsTublin-qF | TGAGGACTGGTGCTTACCGC | Quantifying gene expression in rice |
| OsTublin-qR | GCACCATCAAACCTCAGGGA | |
| AS-F | CTCCCTTCTTGGTTGGTGGG | Marker development for TaWRKY51-2A |
| AS-R | GGAGAAGAAACAATTCAGGCG | |
| B-Hpa11F | TGGCCGCTGCCACTGCTC | CAPS marker development for TaWRKY51-2B |
| B-Hpa11R | CGGTCCCATTCATTCATTCTTT | |
| DGMF | AATAAATTTGTGACTTTCGCAGAAG | CAPS marker development for TaWRKY51-2D |
| DGMR | TTTTTTAACCTCGTCCTGGCA |