Strain | Genotype | Reference |
W303a | MATa, can1–100, his3–11,15, leu2,3,112, trp1–1, ura3–1, ade2–1 | (Sanchez and Lindquist 1990) |
Vector | Plasmid | Gifted from |
Control | pAG305GAL-ccdB | Susan Lindquist (Addgene, Cambridge MA) |
Aβ 1-40 | pAG305GAL- Aβ1-40 | James Shorter (University of Pennsylvania) |
Antibody | Description | Company, Cat# |
α-tubulin | Rabbit monoclonal | Abcam, ab184966 |
β-amyloid | Mouse monoclonal | BioLegend, 803001 |
H3 Total | Mouse monoclonal | Abcam, ab24834 |
H3K4me3 | Rabbit polyclonal | Abcam, ab8580 |
H3K9ac | Rabbit polyclonal | Abcam, ab10812 |
H3S10ph | Rabbit polyclonal | Abcam, ab5176 |
H3K14ac | Rabbit polyclonal | Millipore, 07-353 |
H3K18ac | Rabbit polyclonal | Abcam, ab1191 |
H3K27ac | Rabbit monoclonal | Abcam, ab45173 (Discontinued) |
H3K56ac | Rabbit polyclonal | Active Motif, 39281 |
H3K79me1 | Rabbit polyclonal | Millipore, ABE213 |
H3K79me2 | Rabbit polyclonal | Abcam, ab3594 |
H3K79me3 | Rabbit polyclonal | Abcam, ab2621 |
H4K12ac | Rabbit polyclonal | Abcam, ab46983 |
donkey anti-mouse | Mouse IgG | LI-COR Biosciences, 926-32212 |
donkey anti-rabbit | Rabbit IgG | LI-COR Biosciences, 926-68073 |