Figure EV1. Comparison of mTRPV3 and mTRPV3‐Y564A single‐channel activity and osthole inhibition.

1. Representative single‐channel recordings at 80 mV membrane potential from mTRPV3‐Y564A incorporated into a planar lipid bilayer, in response to 5 μM 2‐APB alone or in combination with 50 μM osthole.
2. Open probability for mTRPV3 and mTRPV3‐Y564A at 80 mV in the presence of agonist 2‐APB and osthole (0 vs. 50 μM osthole: ****P = 0.7 × 10⁻⁵ for mTRPV3 and ****P = 0.4 × 10⁻⁹ for mTRPV3‐Y564A, n = 9 independent experiments). Statistical significance was calculated using one‐way ANOVA followed by Fisher’s least significant difference test. The data presented are mean ± SEM.