Figure 4. Comparison of binding site 2–4 regions in 2‐APB‐ and osthole‐bound structures.

• A–C
  Superposed regions of site 2 (A), 3 (B), and 4 (C) in mTRPV3‐Y564A_2APB (orange) and mTRPV3‐Y564A_osthole (green) structures, with molecules of 2‐APB and osthole shown as ball‐and‐stick models. Residues contributing to ligand binding are shown as sticks. Arrows in C show movement of domains in mTRPV3‐Y564A_osthole compared to mTRPV3‐Y564A_2APB. Residues mutated in functional experiments (Fig 2E) are labeled in red.