FIG 2.

Effect of complementation of the ΔcsrA mutant on growth in mineral medium with different carbon and energy sources. A. baumannii ATCC 19606 + pBAV1K, ATCC 19606 ΔcsrA + pBAV1K, and ΔcsrA + pBAV1K_csrA were grown for 8 h in mineral medium with succinate as the carbon and energy source and in the presence of 50 μg/ml kanamycin. Strains were centrifuged, washed twice with saline (0.9% NaCl), and adjusted with saline to an OD₆₀₀ of 1. Serial dilutions from the cell suspensions (5 μl) were dropped onto solid medium (1.8%) in the presence of the indicated carbon and energy sources and in the presence of 50 μg/ml kanamycin. Growth was monitored after 16 h of incubation at 37°C.