TABLE 2.
Bacterial strains used in this studya
| Strain | Characteristics | Reference |
|---|---|---|
| P. pseudoalcaligenes strains | ||
| CECT5344 | Wild type; uses cyanide as N source | 22 |
| DapA1— | Gm-directed mutant in the dapA1 gene | 30 |
| NitC— | Gm-directed mutant in the nitC gene | 7 |
| Nit1— | Gm-directed mutant in the nit1 gene | This work |
| Nit2— | Km-directed mutant in the nit2 gene | This work |
| Nit4— | Km-directed mutant in the nit4 gene | This work |
| MocR— | Gm-directed mutant in the mocR gene | This work |
| NitC—/ Nit4— | Gm- and Km-directed double mutant in the nitC and nit4 genes, respectively | This work |
| MocR—/ Nit4— | Gm- and Km-directed double mutant in the mocR and nit4 genes, respectively | This work |
| E. coli strains | ||
| DH5α | Lac–; host for most plasmids | 36 |
| S17-1 | Tra+; host for the mobilizable mob plasmids | 45 |
a
Gm, gentamicin; Km, kanamycin.