Fig. 5.

GO and KEGG analysis of DEG. (A) GO classification of the Holstein cow genes. Genes were assigned to GO categories, and the terms were summarized into 3 main GO categories and 61 sub-categories. Light color indicates all genes; deep color indicates DEG. Left Y-axis, percentage of genes; right Y-axis, number of genes. (B) KEGG pathway enrichment analysis of DEG. X-axis represents the number of DEG annotated in the pathway; Y-axis indicates the pathway name. Color of columns represent P-value, based on hypergeometric tests. GO = Gene Ontology; KEGG = Kyoto Encyclopedia of Genes and Genomes; DEG = differentially expressed genes; PI3K = phosphatidylinositol 3 kinase; Akt = RAC-alpha serine/threonine-protein kinase; ECM = extracellular matrix protein; HIF-1 = hypoxia-inducible factor-1; FoxO = forkhead box O; mTOR = mammalian target of rapamycin.