Extended Data Figure 3.

Pore activity during Zymo “full flowcell 1” sequencing runs. (a) Percent of channels that are labeled active throughout zymo bacterial depletion UNCALLED and control runs, based on the percent of signal labeled “pore” or “strand” in the MinKNOW duty times. Curves are smoothed by taking the mean of 92 minute windows, which smooths over mux scans. (b) Number of channels which are “alive” throughout the run, meaning they have the capacity to sequence reads, based on when the last read was produced. This is distinct from the duty time plots in that a channel may not produce a read for several hours but still be considered “alive”.