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. 2021 Oct 12;54(21):3979–3990. doi: 10.1021/acs.accounts.1c00438

Figure 1.

Figure 1

Summary of padlock probe (PLP) ligation mechanism and probe design strategies. (A) Hybridization of PLP to a single stranded DNA (ssDNA) template and ligation mechanism. The phosphorylation of the ligase prior to ligation resorts to ATP or NAD+ as cofactors.11 (B) PLP design allowing ligation to both DNA and RNA sequences. The black horizontal lines in “B” represent a standard phosphodiester bond between two bases. Molecular model in (A) was adapted with permission from ref (18). Copyright 2002 Wiley Online Library.