Table 1.

Primers used during the construction of full-length YFV/RBV chimeric DNAs

Primer	Polarity	Sequence*	Genomic location†
YR1-F	Sense	5′ GAATAAGGGCGACACGGAAATGTC 3′	–
YR1-R	Antisense	5′ CACACAGGATTTACTCGGTTCACTAAACGAG 3′	1–13
YR2-F	Sense	5′ GTTTAGTGAACCGAGTAAATCCTGTGTGC 3′	1–16
YR2-R	Antisense	5′ CATAGCCATCAATCCACCCGTCATCAACAGC 3′	463–493
YR3-F	Sense	5′ CGGGTGGATTGATGGCTATGCAGGTATCCC 3′	474–503
YR3-R	Antisense	5′ GCATCCTTGATCTCCCATGACTCCAGTTGTAAATC 3′	2406–2440
YR4-F	Sense	5′ GAGTCATGGGAGATCAAGGATGCGCCATCAACTTTGG 3′	2418–2454
YR4-R	Antisense	5′ CTGTTATTGAATTCCAGCCACTGACC 3′	7153–7178
YR5-F	Sense	5′ GGTCAGTGGCTGGAATTCAATAACAG 3′	7153–7178
YR5-R	Antisense	5′ TGCCGGCCAGAGGTTTTGTGT 3′	10,829–10,838
YR6-F	Sense	5′ CAAAACCTCTAGCCGGCATGGTC 3′	10,826–10,838
YR6-R	Antisense	5′ GCTGCGCAACTGTTGGGAAG 3′	–

*Sequences are colour-coded: cytomegalovirus promoter, blue; yellow fever virus, black; Rio Bravo virus, red; hepatitis delta virus anti-genomic ribozyme sequence and simian virus 40 polyadenylation signal, green

†Location of the primer binding sites in the chimeric flavivirus genome with numbers starting at the first nucleotide of the flavivirus 5′ untranslated region (not the first nucleotide of the promoter sequence).