Table 2.

Primers used during the construction of full-length ZIKV/RBV chimeric DNAs

Primer	Polarity	Sequence*	Coordinates†
ZR1-F	Sense	5′ GAATAAGGGCGACACGGAAATGTC 3′	–
ZR1-R	Antisense	5′ CAACAACTCGGTTCACTAAACGAGCTCT 3′	1–8
ZR2-F	Sense	5′ CGTTTAGTGAACCGAGTTGTTGATCTGTGTGAATC 3′	1–21
ZR2-R	Antisense	5′ GCCATCAATGCCATAGCTGTGGTCAGC 3′	455–481
ZR3-F	Sense	5′ CACAGCTATGGCATTGATGGCTATGCAGG 3′	461–489
ZR3-R	Antisense	5′ CCCCACATCTCCCATGACTCCAGTTGTAAATC 3′	2398–2429
ZR4-F	Sense	5′ GTCATGGGAGATGTGGGGTGCTC 3′	2412–2434
ZR4-R	Antisense	5′ GGACGGCTGGGGTAATGAAAGTTGTC 3′	6983–7008
ZR5-F	Sense	5′ GACAACTTTCATTACCCCAGCCGTCC 3′	6983–7008
ZR5-R	Antisense	5′ TGCCGGCCAGACCCATGGATTTC 3′	10,724–10,738
ZR6-F	Sense	5′ TGGGTCTGGCCGGCATGGTC 3′	10,732–10,738
ZR6-R	Antisense	5′ CTCAGGGTCAATGCCAGCGCTTAATTTC 3′	–

*Sequences are colour-coded: cytomegalovirus promoter, black; Zika virus, blue; Rio Bravo virus, red; hepatitis delta virus anti-genomic ribozyme sequence and simian virus 40 polyadenylation signal, green.

†Location of the primer binding sites in the chimeric flavivirus genome with numbers starting at the first nucleotide of the flavivirus 5′ untranslated region (not the first nucleotide of the promoter sequence).