Table 4.
Comparison of the in vitro host ranges of ZIKV-RBV(prM-E) and its parental viruses
|
Cell line |
Virus |
||
|---|---|---|---|
|
Rio Bravo virus |
Zika virus |
ZIKV-RBV(prM-E) |
|
|
Mosquito cells |
|||
|
C6/36 |
− |
+ |
+ |
|
CT |
− |
− |
− |
|
Sua 4.0 |
− |
− |
− |
|
Lepidopteran cells |
|||
|
BTI-Tn-5B1-4 |
− |
− |
− |
|
Sf9 |
− |
− |
− |
|
Vertebrate cells |
|||
|
DEF |
+ |
+ |
+ |
|
LLC-MK2 |
+ |
+ |
+ |
|
Murine microglia |
− |
− |
− |
|
Vero |
+ |
+ |
+ |
The chimeric virus had been passaged twice in Vero cells prior to the experiment. The first passage cultures were inoculated with virus at an m.o.i. of 0.1. A total of five passages were performed. A cell line was considered to support virus replication if RT-PCR products were detected after every passage.