REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD25-FITC	Miltenyi Biotec	Cat. No. 130-113-283, RRID:AB_2734062
CD3-PECy7	BD Biosciences	Cat. No. 557851, Research Resource Identifier:AB_396896
CD69-APC	BD Biosciences	Cat. No. 555533, Research Resource Identifier:AB_398602
IgG Compbeads	BD Biosciences	Cat. No. 552843
Biological samples
HEK293 T Lenti-X cells	n/a	n/a
Chemicals, peptides, and recombinant proteins
Antibiotic/antimycotic solution	Wisent Bio Products	Product No. 450-115-EL
Basic fibroblast growth factor (bFGF), human, recombinant	STEMCELL Technologies	Cat. No. 78003.2
D-firefly luciferin potassium salt	Perkin Elmer	Part No. 122799
DMEM, high glucose with pyruvate	Thermofisher Scientific	Cat. No. 11995073
DNase vial (D2)	Worthington Biochemical Corporation	Cat. No. NC9216647
EDTA	Millipore Sigma	Product No. 20158
Epidermal growth factor (EGF), human, recombinant	STEMCELL Technologies	Cat. No. 78006
Fetal bovine serum (FBS)	Wisent Bioproducts	Product No. 098-150
Heparin solution	STEMCELL Technologies	Cat. No. 07980
Liberase research grade	Millipore Sigma	Product No. LIBTM-RO
MEM-non-essential amino acids	Thermofisher Scientific	Cat. No. 11140050
MycoZap prophylactic	Lonza	Cat. No. VZA-2031
NeuroCult NS-A proliferation kit (Human)	STEMCELL Technologies	Cat. No. 05751
Nonidet 40 (NP40)	Thermofisher Scientific	Cat. No. 98379
PBS pH 7.4, with calcium and magnesium	Wisent Bio Products	Product No. 311-011-CL
Polybrene infection/transfection reagent	Millipore Sigma	Product No. TR-1003
PrestoBlue reagent	Thermofisher Scientific	Cat. No. A13262
Prime-XV T cell expansion media XSFM (XSFM)	Irvine Scientific	Cat. No. 91141
Trypan blue	Thermofisher Scientific	Cat. No. 15250-061
TrypLE™ express enzyme (1×), phenol red	Thermofisher Scientific	Cat. No. 12605028
7-AAD viability dye	Beckman Coulter	Product No. A07704
Critical commercial assays
Cytometric bead array (CBA) Kit	BD Biosciences	Cat. No. 550749
Experimental models: Cell lines
CAR-T cells	Please refer to Vora et al. (2020)	n/a
Patient-derived GBM stem cell lines	Dr. Sheila Singh’s Laboratory	n/a
Software and algorithms
Kaluza Analysis Software	Beckman Coulter	Web Link: https://www.mybeckman.ca/flow-cytometry/software/kaluza
FLUOstar omega fluorescence 556 microplate Reader	BMG Labtech	n/a
Other
Countess chamber slides	Invitrogen	Cat. No. C10228
5 mL round-bottom polystyrene tubes with 35 μm strainer lid	Corning	Cat. No. CA21008-948