REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human anti-CD98 (20 μg/mL for 5×104 cells)	Absolute Antibody	Ab00361–10.0
Goat anti-Human IgG DyLight 650 (6.25 μg/mL for 5×104 cells)	ThermoFisher	SA5-10137; RRID:AB_2556717
Goat anti-Human IgA FITC (1:1000 dilution for 5×104 cells)	ThermoFisher	A18788; RRID:AB_2535565
Mouse anti-Human IgM BV650 (1:40 dilution for 5×104 cells)	BioLegend	314526; RRID:AB_2563836
Critical commercial assays
Live/Dead Aqua	ThermoFisher	L34957
Anti-murine IgG Kappa Beads for compensation	BD Biosciences	552843
ArC Amine-reactive beads for compensation	ThermoFisher	A10346
Biological samples
Human plasma and serum samples (age range 24–92 yrs; 52% male)	Wong et al. (2021)	https://doi.org/10.1016/j.xcrm.2021.100321
Experimental models: Cell lines
HULEC-5a	ATCC	CRL-3244; RRID:CVCL_0A11
Chemicals, peptides, and recombinant proteins
TrypLE Express	ThermoFisher	12604021
PBS 1×	Lonza	7–516F
MCDB 131 medium	ThermoFisher	10372019
Epidermal Growth Factor	Sigma Aldrich	E5036-500UG
Hydrocortisone	StemCell Technologies	07925
L-glutamine	Sigma Aldrich	G7513-100ML
Fetal Calf Serum	ThermoFisher	10099
Paraformaldehyde (PFA)	Electron Microscopy Sciences	15710
Deposited data
Source data	Wong et al. (2021)	https://data.mendeley.com/datasets/k33vwbdfsn/3
Other
96-well U bottom plate	Corning	3799
T175 Flask	Corning	431306
50 mL Tube	Corning	352070
70 μm Cell Strainer	Corning	352350
Flow Cytometer	BD Biosciences	LSRFortessa