Table 7.
Zinc level | 0 | 30 | 120 | 0 | 30 | 120 | SEM | Main effect | Zn level |
LPS |
P-value |
|||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
LPS | - | - | - | + | + | + | 0 | 30 | 120 | - | + | Zn level | LPS | Interaction | ||
CLDN-1 | 1.00c | 1.27b | 1.50a | 0.66d | 0.80d | 1.33b | 0.083 | 0.83c | 1.03b | 1.41a | 1.26a | 0.93b | <0.001 | <0.001 | 0.046 | |
CLDN-2 | 1.01 | 0.84 | 0.85 | 1.32 | 1.22 | 0.92 | 0.040 | 1.12a | 1.04b | 0.81b | 0.90b | 1.08a | 0.001 | <0.001 | 0.084 | |
OCLD | 1.01 | 1.20 | 1.44 | 0.73 | 0.85 | 1.34 | 0.045 | 0.87c | 1.02b | 1.39a | 1.27a | 0.97b | <0.001 | <0.001 | 0.163 | |
ZO-1 | 1.01c | 1.25b | 1.67a | 0.68d | 1.18b | 1.56c | 0.052 | 0.84c | 1.21b | 1.62a | 1.31a | 1.14b | <0.001 | <0.001 | 0.029 | |
ZO-2 | 1.00 | 1.06 | 1.11 | 0.93 | 1.04 | 1.02 | 0.028 | 0.97 | 1.05 | 1.07 | 1.06 | 1.00 | 0.291 | 0.264 | 0.846 | |
ZO-3 | 1.01 | 1.49 | 1.63 | 0.69 | 1.17 | 1.46 | 0.050 | 0.85c | 1.33b | 1.55a | 1.37a | 1.11b | <0.001 | <0.001 | 0.208 | |
EPCAM | 1.01 | 1.07 | 1.10 | 0.75 | 0.94 | 0.95 | 0.030 | 0.88 | 1.00 | 1.02 | 1.06a | 0.88b | 0.068 | 0.002 | 0.594 | |
JAM2 | 1.01 | 1.19 | 1.08 | 0.81 | 0.84 | 1.06 | 0.032 | 0.91 | 1.02 | 1.07 | 1.10a | 0.90b | 0.063 | 0.001 | 0.067 | |
JAM3 | 1.00 | 1.11 | 1.10 | 0.89 | 0.98 | 1.07 | 0.031 | 0.95 | 1.05 | 1.09 | 1.07 | 0.98 | 0.187 | 0.148 | 0.831 | |
MLCK | 1.01d | 0.76d | 0.65d | 3.95a | 3.50b | 2.31c | 0.198 | 2.48a | 2.13b | 1.48c | 0.81b | 3.25a | <0.001 | <0.001 | <0.001 |
Abbreviations: CLDN-1, claudin1; CLDN-2, claudin 2; EPCAM, epithelial cell adhesion molecule; JAM2, junctional adhesion molecule-2; JAM3, junctional adhesion molecule-3; MLCK, myosin light-chain kinase; OCLD, occluding; ZO-1, zonula occludens-1; ZO-2, zonula occludens-2; ZO-3, zonula occludens-3.
Mean values in a row without common superscript are significantly different (P < 0.05) (n = 8).