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. 2021 Oct 26;13(10):1595–1609. doi: 10.4252/wjsc.v13.i10.1595

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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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Gene expression analysis in HepaRG cells at passage 10 or passage 20 in basal conditions or after the transdifferentiation protocol (dimethyl sulfoxide). A: mRNA level of albumin; B: Cytochrome P350 3A4 (CYP3A4); C: Gamma-glutamyl transpeptidase (γ-GT); D: Carcinoembryonic antigen (CEA) measured by reverse transcription-polymerase chain reaction. Data in the graphs are represented as the mean ± standard deviation of three independent experiments. Statistical differences were assessed by two-way analysis of variance followed by the Tukey test as the post hoc test. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. P10: Passage 10; P20: Passage 20; DMSO: Dimethyl sulfoxide.