Table 1.
Small interfering RNA sequences
|
|
Forward
|
Reverse
|
| siGlut1-1 | 5′-CACCGGGAGTGACAAAGACTTTGTTCAAGCA-3′ | 5′-GATCCAAAAAAGGGAGTGACAAAGACTTCTC-3′ |
| Negative control | 5′-ATCCGACTTCATAAGGCGCATGCT-3′ | 5′-AGTATTCCGCGTACGAAGTTCTGC-3′ |
| siRNA Smad4 targeting four different sequences1 | ||
| GCAAUUGAAAGUUUGGUAA, CCCACAACCUUUAGACUGA, GAAUCCAUAUCACUACGAA and GUACAGAGUUACUACUUAG | ||
| siRNA Smad2/31 | Sense | Antisense |
| sc-37239A | CUUGCUGGAUUGAACUUCAtt | UGAAGUUCAAUCCAGCAAGtt |
| sc-37239B | CCGUCGUAGUAUUCAUGUAtt | UACAUGAAUACUACGACGGtt |
| sc-37239C | CUGACUCCUUGUUUAAUGAtt | UCAUUAAACAAGGAGUCAGtt |
| sc-37239D | GGAAGCUGAGAGUUAUAGAtt | UCUAUAACUCUCAGCUUCCtt |
1
Purchased from Santa Cruz Biotechnology. sc-37239: Smad2/3 siRNA (m) is a pool of four different siRNA duplexes.