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. Author manuscript; available in PMC: 2022 Oct 21.
Published in final edited form as: Mol Cell. 2021 Aug 25;81(20):4300–4318.e13. doi: 10.1016/j.molcel.2021.07.042

Figure 2. CircRNAs containing eGFP(+) oligo have higher cap-independent translation activity.

Figure 2.

(A) A schematic of the circRNA polysome profiling for translating circRNAs. (B) (Poly)ribosome fractionations of cells transfected with oligo library split-eGFP circRNA reporter followed by CHX treatment. (C) Quantification of the percentage of (poly)ribosome-enriched oligos of captured eGFP(−) or eGFP(+) oligos. (D) Sequencing reads from Ribo-seq and QTI-seq plotted on the genes showing eGFP(+) oligos harboring aTIS, nTIS, and dTIS with overlapping annotated circRNAs (brown segments). (E) Quantification of the percentage of eGFP(−) or eGFP(+) oligos harboring no TIS (TIS(−)) (left) or more than one TIS (TIS(+)) (right), and the percentage of aTIS, nTIS, or dTIS oligos among eGFP(+)/TIS(+) oligos.