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The expression of the optimized sequences designed with codon usage and codon-pair context in Pichia pastoris. A, C The expression analysis of MT1-MMP E2C6 and ADAM17 A9B8 by Western blot using anti-his tag primary antibody. B, D The quantitative analysis to A, C. #1, #2 and #3 represent three different recombinant strains. M protein marker, CUB codon usage bias, CPB codon pair bias
