Table 2.
Fourteen novel targets validated by RLM-5′ RACE assay for nine wheat or pooideae specific miRNAs
| miRNA | miRNA conservation | Target gene ID | Target gene annotation |
|---|---|---|---|
| wheat_miR1356 | tae | TRIAE_CS42_6BS_TGACv1_516613_AA1675760.1 | mTERF, mitochondrial transcription factor |
| TRIAE_CS42_6DS_TGACv1_543352_AA1738780.2 | mTERF, mitochondrial transcription factor | ||
| TRIAE_CS42_6DS_TGACv1_543340_AA1738640.3 | mTERF, mitochondrial transcription factor | ||
| TRIAE_CS42_6BS_TGACv1_514840_AA1664920.1 | mTERF, mitochondrial transcription factor | ||
| miR9662a-3p | tae | TRIAE_CS42_5DS_TGACv1_458236_AA1492830.1 | mTERF, mitochondrial transcription factor |
| TRIAE_CS42_6BS_TGACv1_513268_AA1636720.1 | mTERF, mitochondrial transcription factor | ||
| miR9772 | tae, ata | TRIAE_CS42_6BS_TGACv1_513312_AA1637980.1 | F-box domain containing protein |
| TRIAE_CS42_6BS_TGACv1_518580_AA1677490.1 | F-box domain containing protein | ||
| miR9674b-5p | tae, ata | TRIAE_CS42_6BS_TGACv1_513439_AA1641610.4 | Protein Rf1. PPR repeat domain containing protein |
| miR7757-5p | tae, bdi | TRIAE_CS42_6AS_TGACv1_485261_AA1541860.2 | disease resistance protein RGA5-like |
| miR9676-5p | tae | TRIAE_CS42_3B_TGACv1_224755_AA0801120.1 | biostress-resistance-related protein |
| wheat_miR259-5p | tae | TRIAE_CS42_2DS_TGACv1_179410_AA0606910.1 | Kinesin-like protein KIF15 |
| wheat_miR652-3p | tae | TRIAE_CS42_4DS_TGACv1_361398_AA1167080.1 | protein FAF-like, chloroplastic |
| miR5048-5p | tae, hvu | TRIAE_CS42_6AS_TGACv1_485227_AA1540680.1 | putative terpene synthase |
Note: tae, ata, bdi and hvu design Triticum aestivum, Aegilops tauschii, Brachypodium distachyon and Hordeum vulgare, respectively