Table 2.
Primers used in this work
| Primer | Sequence (5′ - > 3′) |
|---|---|
| Mutant and overexpressing strains | |
| CV3905-del1 | TAGGGCCCGAAACTGCGGCTCGAACGCC |
| CV3905-del2 | ATCAAGCTTCATCGAAAGAGTCCCCGTGC |
| CV3905-del3 | ATCAAGCTTGAAAACACGCCGGCCTGAGC |
| CV3905-del4 | TTGGATCCCTGGTGGCGGGCTTTGTTGC |
| CV_3905-Comp-Fw | GGTACCCTGCAGAGCCAGTCCGCATTAAGGCG |
| CV_3905-Sup-Rv | GGTTACGAGCTCGACGCTCAGCGTCAGAGCTG |
| Northern blot | |
| CV0209-NB-Fw | TGCAGGTGAAGTTGAGCACCCC |
| CV0209-NB-Rv | CCGATGAAGCAGGCGGAATAGCC |
| NB CV2534 -Fw | GATGGTGCTGATCGCGATGG |
| NB CV2534 -Rv | CCAGGGTCTCGATCAGCATC |
| NB CV2541-Fw | CCATTCTGTCCGCGCTGTTG |
| NB CV2541-Rv | GCATTTCGTCCATGTCGCGG |
| NB CV3659-Fw | CACCTCTGGTGCAGGCTTTC |
| NB CV3659-Rv | GACAGCGACTGGGTGAACTG |
| NB CV0568-Fw | ACGCCAAGCTGGGACTCAAC |
| NB CV0568-Rv | GCTCTACCTCCCGTTCGATG |
| EMSA | |
| pCV0209-Fw | TTGGATCCTGCCGGAGCGGAATGCAACT |
| pCV0209-Rv | ATTACTGCAGGCGGTGGCTTCGGCGGTATAC |
| pCV3905-Fw | GGTACCCTGCAGAGCCAGTCCGCATTAAGGCG |
| pCV3905-Rv | ATCAAGCTTCATCGAAAGAGTCCCCGT |
| pCV0210-Fw | CTCGCTGGCCGATCTGGGAC |
| pCV0210-Rv | GGCGGAATACAGCGCGAAGC |
| pCV1486Fw | GACCAAGTGATAGACGCCGG |
| CV1486-DEL2 | GGCCTAAAGCTTCTCGGTCATGAGGATGTCTG |
| pCV2087Fw | CCTCGTAAAAGCGGATGGCG |
| pCV2087Rv | CGTCGAAACCCTTCATCGCG |
| pCV2534Fw | GATCACCGACACCCTCAAGC |
| pCV2534Rv | GCGACAACACCAGCAGCTTG |
| pCV2545Fw | TGACTCTCCCCCATTGGGAG |
| pCV2545Rv | ATCGGGATGCCCAGCACCGC |
| pCV3659Fw | GACCGACAATGACGGCTACG |
| pCV3659Rv | GCCGAAATGCAGCACGAAAG |
| CV0208 Fw | GACCATACTAGACAGCTACGCC |
| CV0208Rv | CAGCGTGGTGATTTGCGGATAG |
Underlined letters indicate restriction enzyme recognition sites used for cloning purposes.