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. 2021 Nov 4;19:163. doi: 10.1186/s12958-021-00845-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The mRNA expression analysis in blastocysts obtained in ICSI group, ICSI-AOA group and dICSI-AOA group. A Heatmap of global gene expression. Letters and numbers denote ID of a specific embryo. B Venn diagram with differentially expressed genes (DEGs) for pair-wise comparisons (ICSI-AOA vs. ICSI, dICSI-AOA vs. ICSI, ICSI-AOA vs. dICSI-AOA). C Volcano plot defined up-regulated/down-regulated mRNAs for pair-wise comparisons (ICSI-AOA vs. ICSI, dICSI-AOA vs. ICSI). D Principal Component Analysis (PCA) of the RNA-Seq samples: ICSI (red), ICSI-AOA (yellow) and dICSI-AOA (green)