Fig. 1.

Representative flow cytometry gating strategy for the broad spectrum panel. A To identify immune subsets, non-relevant events including debris, doublets, red blood cells, and dead cells were removed through gating using the Flowjo software. Gating on CD45 cells, B immune cells were then segregated into (1) B cells, (2) T cells, and (3) innate immune cells (non B and T cells). C Gating on the innate population from B3, cells were divided into neutrophils and monocyte (MO) subsets: classical, intermediate, and activated, which were gated off of total monocytes