Table 1A.
LINC00511, miR-185-3p, and miR-301a-3p primer sequences used for qPCR versus GAPDH and U6, respectively.
| Target ID | Primer | Location | Amplicon size | Sequence (5` to 3`) |
|---|---|---|---|---|
| LINC00511 | Forward | 121–141 | 178 nt | 5`-CTGTTTGGACGTGGTGAGGA-3` |
| Reverse | 319–338 | 5`-CCCTTCAGTTCATGACGCCT-3` | ||
| GAPDH | Forward | 155–173 | 66 nt | 5`-AGCCACATCGCTCAGACAC-3` |
| Reverse | 220–202 | 5`-GCCCAATACGACCAAATCC-3` | ||
| miR-185-3p | Forward | 1–20 | N.A. | 5`-GGGGCTGGCTTTCCTCTGG-3` |
| Reverse | N.A. | |||
| miR-301a-3p | Forward | 1–22 | N.A. | 5`-CAGTGCAATAGTATTGTCAAA-3` |
| Reverse | N.A. | |||
| U6 | Forward | 1–23 | 70 nt | 5`-ATTGGAACGATACAGAGAAGATT-3` |
| Reverse | 5`-GGAACGCTTCACGAATTTG-3` |
nt, nucleotides; N.A., not available to researchers, Intellectual property of Qiagen Co.