. Author manuscript; available in PMC: 2021 Nov 4.

Published in final edited form as: J Vis Exp. 2019 Aug 21;(150):10.3791/59901. doi: 10.3791/59901

Table 1.

List of primers.

Primer	Sequence
3′ linker 1	rAppCTGTAGGCACCATCAAT–NH2
lower size marker	rArUrCrGrCrArUrGrCrUrGrArCrGrUrArCrUrArGGTAACCGCATCATGCGTC
upper size marker	rArArUrCrArGrCrGrGrArUrUrGrCrArUrGrArArCrGrUrArCrArUrArGGTAACCGCATCATGCGTC
barcode1	/5AmMC6/ACGCTCTTCCGATCTrArGrCrG
barcode2	/5AmMC6/ACGCTCTTCCGATCTrCrGrUrC
barcode3	/5AmMC6/ACGCTCTTCCGATCTrCrUrGrG
barcode4	/5AmMC6/ACGCTCTTCCGATCTrArCrUrU
barcode5	/5AmMC6/ACGCTCTTCCGATCTrGrGrGrU
barcode6	/5AmMC6/ACGCTCTTCCGATCTrGrUrUrA
barcode7	/5AmMC6/ACGCTCTTCCGATCTrUrArUrG
barcode8	/5AmMC6/ACGCTCTTCCGATCTrUrCrGrC
barcode9	/5AmMC6/ACGCTCTTCCGATCTrGrCrArG
barcode10	/5AmMC6/ACGCTCTTCCGATCTrArUrArC
barcode11	/5AmMC6/ACGCTCTTCCGATCTrUrUrCrU
barcode12	/5AmMC6/ACGCTCTTCCGATCTrCrArArU
barcode13	/5AmMC6/ACGCTCTTCCGATCTrArArGrA
barcode14	/5AmMC6/ACGCTCTTCCGATCTrUrGrArA
barcode15	/5AmMC6/ACGCTCTTCCGATCTrUrGrGrG
barcode16	/5AmMC6/ACGCTCTTCCGATCTrArUrUrG
barcode17	/5AmMC6/ACGCTCTTCCGATCTrUrCrArU
barcode18	/5AmMC6/ACGCTCTTCCGATCTrGrUrArU
RT primer	ATTGATGGTGCCTACAG
PCR primer F	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
PCR primer R	CAAGCAGAAGACGGCATACGAGCTCTTCCGATCTATTGATGGTGCCTACAG