Figure 4.

Effect of HS on the morphology structure of colon. CON indicates no heat stress group, HS1, 7, 14, 21 indicate heat stress day 1, 7, 14, and 21 groups respectively, n = 6; “**” indicates p < 0.01, “****” means p < 0.0001. (A) HE and PAS staining results of paraffin sections of colon; (B) villus height/crypt depth; (C) PAS positive cell number; (D) intestinal muscle layer thickness. As the arrows showing, after HS treatment, the colonic mucosal were loosely arranged and showed signs of bleeding on days 1, 7 and 14 of HS, the local injury and lymphatic infiltration also had been found on days 7 and 14 of HS.