Figure 1. Growth and NO${}_3^{-}$ reduction in planktonic mono-cultures.

(A) Growth under planktonic anoxic conditions with 25 mM NO${}_3^{-}$ and 0.3% methanol at 30 °C. (triplicate cultures). (B and C) Growth rates and NO${}_3^{-}$ reduction rates, respectively, at different NO${}_3^{-}$ concentrations. The curves in (B) are derived from non-fit linear regression; the growth rates at 107 mM in the strain NL23 cultures were not included. Each point is the average of three to eight replicate anoxic cultures. (D) Specific NO${}_3^{-}$ reduction rates. These rates were calculated with the NO${}_3^{-}$ reduction rates by the generated culture biomass (OD_600 nm) at the end of the exponential phase. Each point represents data from triplicate cultures. Data for strain JAM1 in C and D were taken from Geoffroy et al. (2018).