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. 2021 Nov 1;9:e12424. doi: 10.7717/peerj.12424

Figure 2. Dynamics of denitrification of the planktonic co-cultures.

Figure 2

(A and D) Strain JAM1 and strain NL23 were co-cultured in the 0.5% NaCl Methylophaga 1403 medium under anoxic conditions with 21.4 mM NO3− and 0.3% methanol at 30 ° C. As controls, mono-cultures were carried out with both strains under the same conditions (C and F). Strain NL23 inoculum was from pre-cultures cultured under oxic conditions (no NO3−; A and C) or under anoxic conditions (with NO3−; D and F). The growth (OD 600 nm) and the concentrations of NO 3− and NO2− were measured. Results are from triplicate cultures and representative of three sets of triplicate assays. (B and E) Concentrations at the end of the culture assays of strain JAM1 and strain NL23 determined by qPCR with primers targeting napA for strain NL23 and narG1 for strain JAM1. Results in (B) are from the non-stimulated NL23 co-cultures (A), and, in (E), from the stimulated NL23 co-cultures (C). Results are from triplicate cultures.