Table 1. Operating conditions of the reactors.

Medium	NaCl	Methanol	NO3−	Biofilm cultures
	% w/v	% v/v	mM	Reactors 1, 2	Reactor 3
Methylophaga 1403	0	0.3	21.4	ND	NL23
Methylophaga 1403	0.5	0.1	7.14	Co-culture	ND
Methylophaga 1403	0.5	0.2	14.3	Co-culture	ND
Methylophaga 1403	0.5	0.3	21.4	Co-culture*	NL23*
Methylophaga 1403	1.0	0.3	21.4	Co-culture	NL23
Methylophaga 1403	2.0	0.3	21.4	Co-culture	NL23*
Methylophaga 1403	2.75	0.3	21.4	Co-culture*	NL23
IO + supplements		0.3	21.4	Co-culture	NL23
IO without supplements		0.3	21.4	Co-culture*	NL23

Notes.

Reactors were run with successive changes of medium following the order in the Table, with one exception: Reactor 1 was run with the Instant Ocean (IO) medium but without supplements first, and then supplements were reintroduced.

^*Supports taken for RNA extraction in reactors 2 and 3.

ND, not done.