Table 2. Sequences of the oligonucleotides used for qPCR and RT-qPCR assays.
| Name | Sequence 5′–3′ | Th ∘C | Length bp |
|---|---|---|---|
| Standards for qPCR | |||
| Strain NL23 | |||
| napA-1171f | TACAACGTCCACCTGCTGAC | 55 | 625 |
| napA-1846r | TCCGCTTCGTGGTTTTCGTA | ||
| Strain JAM1 | |||
| narG1-G | ATGACAAGATCGTGCGTTCT | 57 | 664 |
| narG1-D | GGTGTACGGGTCATTGGTAAG | ||
| qPCR | |||
| Strain NL23 (napA) | |||
| napA-1415f | AGGACGGGCGGATCAATTTT | 61 | 131 |
| napA-1526r | CGGATATGCATCGGACACGA | ||
| Strain JAM1 (narG1) | |||
| narG-1313f | AGCCCACATCGTATCAAGCA | 61 | 149 |
| narG-1461r | CCACGCACCGCAGTATATTG | ||
| RT qPCR | |||
| Strain NL23 | |||
| napA-1415f | AGGACGGGCGGATCAATTTT | 61 | 112 |
| napA-1526R | CGGATATGCATCGGACACGA | ||
| nirKf | CGCACAACATCGACTTCCA | 61 | 130 |
| nirKr | GCGCAGTGATAGACGAAAAC | ||
| rpoBf | GCCATCAACAAGCAGTACGA | 61 | 128 |
| rpoBr | GCCACGAAGACCTTGACCAT | ||
| dnaGf | CCCGATCAAAACGCCAAGTA | 61 | 141 |
| dnaGr | CGCATCCATGTAGCCTTCGA | ||
| Strain JAM1 | |||
| narG1-1313f | AGCCCACATCGTATCAAGCA | 60 | 149 |
| narG1-1461r | CCACGCACCGCAGTATATTG | ||
| narG2-597f | TTACGCTGCAGGATCACGTT | 60 | 127 |
| narG2-723r | TGACTCGGGTACATCGGTCT | ||
| rpoB-3861f | TGAGATGGAGGTTTGGGCAC | 60 | 146 |
| rpoB-4006r | GCATACCTGCATCCATCCGA | ||
| dnaG-774f | CATCCTGATCGTGGAAGGTT | 60 | 121 |
| dnaG-894r | GCTGCGAATCAACTGACGTA) |
Notes.
Th, hybridization temperature.