Figure 4.

SOD activation reduces heat stress-induced ferroptosis-like death of GMECs. Adding a certain concentration of SOD activator CuCl₂ (15, 30 mM) to heat-stressed GMECs. After 24 h of co-cultivation, the degree of cell ferroptosis-like death can be judged by detecting cell viability, iron ion concentration, and ROS level: (A) SOD determination kit was used to determine the activity of SOD. (B) Western blotting was used to measure the expression of GPX4 protein. (C) MTT assay was used to detect the cell viability. (D) Determination of ROS content. (E) Determination of iron ion concentration by spectrophotometer. *P < 0.05 versus control, n=6.